Injection Site Reactions
A PATENTED HUMAN-BASED PLATFORM TO ADDRESS INJECTION SITE REACTIONS AT THE ORGAN, CELLULAR & MOLECULAR LEVELS.
EX-VIVO HUMAN SKIN MODEL
Immunocompetent and injectable living human skin model to study tissue immune response.
HUMAN PRIMARY MAST CELLS
Functional human primary cells in culture to study mast cells degranulation.
MAST CELLS RECEPTOR MRGPRX2
Complementary cell lines assay to evaluate the activation of human MRGPRX2.
A UNIQUE INJECTABLE EX-VIVO HUMAN SKIN MODEL TO BETTER UNDERSTAND INJECTION SITE REACTION.
Upon injection of a therapeutic molecule or formulation in a standardized healthy and immunocompetent human skin model, we analyse mast cells degranulation, cytokine release & perform mRNA sequencing to precisely characterize the type of inflammatory reaction we are encountering.
Multiple donors of different age, gender and ethnicity.
Parallel testing of different compounds in the same donor.
Artificial intelligence-assisted MCs degranulation scoring.
Positive & negative controls.
Triplicate conditions.
MCs granules stained with conjugated avidin.
DAPI counter-staining.
Fluorescent microscopy.
AI-assisted degranulation scoring and image processing.
Mast cells degranulation observed on a full-thickness epidermis & dermis human skin sample after subcutaneous injection of IL-1R antagonist (Avidin & DAPI).
HUMAN PRIMARY MAST CELL ASSAYS TO BETTER UNDERSTAND DEGRANULATION.
Immune reaction can result from mast cells triggered either directly or indirectly.
Genoskin mast cells assays are performed on connective tissue-type human primary cells in culture to generate more relevant human data and support data previously obtained in our injectable skin model.
Assays performed on functional human primary mast cells in culture provide a better understanding of the pathways through which these immune cells are triggered.
Connective tissue-type mast cells production and assays workflow: Peripheral blood obtained from healthy adult donors. In a 3-month process, we are able to obtain mature mast cells that show the same metabolism and expression as in the human body.
2 weeks lead time.
High throughput screening.
Multiple formulations tested per donor.
Connective tissue-type mast cells in culture.
Human primary connective tissue-type mast cells cultured for 3 months and stained with fluorescent avidin & DAPI.
COMPLEMENTARY CELL-LINE ASSAYS TO EVALUATE MRGPRX2 ACTIVATION.
Complementary cell-line assays on cells expressing MRGPRX2 based on Genoskin proprietary platform allows formulation optimization or screening of new drug-candidate.
High throughput screening.
Target specifically MRGPRX2.
