Scientific poster

Single-cell profiling of biostabilized natural human skin identifies mast cells as an important target of injected mRNA-1273 COVID-19 vaccine.

Research by M. Scholaert1,2, M. Peries2, E. Braun2, K. Polak2, J. Martin1, N. Serhan1, A. Loste1, A. Bruner1, L. Basso1, B. Chaput3, E. Merle4, E. Pagès2 and N. Gaudenzio1,2

1Toulouse Insitute for Infectious and Inflammatory Diseases (Infinity) INSERM UMR1291 – CNRS UMR5051 – University Toulouse III, France, 2Genoskin SAS, Toulouse, France, 3Department of Plastic, Reconstructive and Aesthetic Surgery, Rangueil Hospital, CHU Toulouse, Toulouse, France, 4Genoskin Inc, Salem MA, USA

The field of vaccination is witnessing a remarkable surge in innovative strategies aimed at enhancing effectiveness while minimizing local side effects.

There is a need to develop technological platforms capable of generating human data prior to progressing to clinical trials. Our research introduces a flexible solution designed for the comprehensive monitoring of the natural human skin ecosystem’s response to vaccines over time.

METHOD: Our platform leverages bioengineering techniques to repurpose surgical resections, enabling a comprehensive analysis of vaccine responses at both organ and single-cell levels. This method allows us to track the presence of the vaccine Spike mRNA in various immune and structural cells within the skin.

KEY FINDINGS: Upon injection of the mRNA-1273 COVID-19 vaccine, we observed significant levels of vaccine mRNA in mast cells and, to a lesser extent, in DC/macrophages. This was consistent across different administration routes, indicating a natural affinity of LNP-loaded mRNA vaccines for these cell types. The findings suggest that mast cells play a critical role in the skin’s response to mRNA vaccines.
This study highlights the importance of mast cells in response to the mRNA-1273 COVID-19 vaccine. This insight provides a multiscale vision of skin vaccination, offering direct translational relevance and informing the development of new vaccination strategies.

This poster was presented at the European Mast cell & Basophil Research Network meeting 2024.

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