Hyposkin®
Biostabilized ex vivo human skin model
A versatile ex vivo human skin model
designed for testing injectable therapeutics
Biostabilized & functional
- viable and functional for 7+ days
- immunocompetent
- contains 14 immune cell types naturally presented in human skin
Multiple administration routes
- subcutaneous & intradermal injections
- topical & transdermal applications
- systemic-like administration
Cutting-edge data analysis for relevant results
Get the whole picture before you move forward
To help you obtain the full picture of how skin and immune cells respond to your formula, our expertise includes unbiased data analysis via artificial intelligence, spatial biology for specific applications.
Biological & immunological validation
Human skin structure and its resident immune system are maintained for 7 days
Since we use real human skin to generate HypoSkin®, it has the same structure and composition as in vivo human skin. Hematoxylin & Eosin staining confirms the preservation of tissue and cellular structures across all three skin layers (epidermis, dermis, and hypodermis). Visual examination reveals a mature stratum corneum, a dermal-epidermal junction mirroring real human skin, and intact basal layers and rete ridges. Notably, the images also demonstrate the sustained integrity of HypoSkin® for a duration of up to 7 days.
Presence of skin-resident immune cells at 7 days
Genoskin’s proprietary technology enables the maintenance of all skin-resident immune cells for over a week during experimentation.
Figure 1 shows immune cells are characterized in HypoSkin® using the MANTIS® spatial biology platform (myeloid panel: Langerhans, Dendritic cells, Mast cells, Macrophages…). Figure 2 shows the cell distribution across different population over time (colored based on the day of sampling).
Functional and activatable skin-resident human immune cells
HypoSkin® remains fully living and immunocompetent throughout a 7-day experimental period. This enables longitudinal secretome analysis for evaluating the toxicity and efficacy of therapeutic and chemical compounds.
Fold change of cytokines concentrations in clinical-grade water vs vaccine injected HypoSkin® skin model after 8h and 24h in 6 different donors.
Scientific publications using HypoSkin®
See how others use the HypoSkin® model to generate results
August 2022 - Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology
- Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology
- Published in Molecular Therapy Methods & Clinical Development – VOLUME 26, P441-458, SEPTEMBER 08, 2022
- Aurelien Lathuiliere, Remi Vernet, Emily Charrier, Muriel Urwyler, Olivier Von Rohr, Marie-Claude Belkouch, Valentin Saingier, Thomas Bouvarel, Davy Guillarme, Adrien Engel, Patrick Salmon, Thomas Laumonier, Julien Grogg, Nicolas Mach
- Published in Molecular Therapy Methods & Clinical Development – VOLUME 26, P441-458, SEPTEMBER 08, 2022
November 2021 - A suspended layer additive manufacturing approach to the bioprinting of tri-layered skin equivalents
- A suspended layer additive manufacturing approach to the bioprinting of tri-layered skin equivalents
- Published in APL Bioengineering – 2021 Nov 30;5(4):046103.
- Richard J A Moakes, Jessica J Senior, Thomas E Robinson, Miruna Chipara, Aleksandar Atansov, Amy Naylor, Anthony D Metcalfe, Alan M Smith, Liam M Grover
- Published in APL Bioengineering – 2021 Nov 30;5(4):046103.
July 2021 - Poster - Mitigation of Injection Site Reactions after Subcutaneous Administration of Dalcinonacog Alfa (DalcA) in Hemophilia B Using Preclinical Models
- Mitigation of Injection Site Reactions after Subcutaneous Administration of Dalcinonacog Alfa (DalcA) in Hemophilia B Using Preclinical Models
- Scientific Poster – ISTH 2021 Congress – Abstract PB0453
- N. Le Moan, L. Kelly, E. Merle, P. Descargues, N. Gaudenzio, H. Gagnon, A. Chatterji, G.E. Blouse
- Scientific Poster – ISTH 2021 Congress – Abstract PB0453
July 2021 - Poster - Assessing subcutaneous injection site reactions by leveraging immunocompetent human skin and artificial intelligence
- Assessing subcutaneous injection site reactions by leveraging immunocompetent human skin and artificial intelligence
- Scientific Poster – CRS 2021 Virtual Annual Meeting – July 25 to 29, 2021.
- Emeline Pagès, Emilie Braun, Eric Merle, Pascal Descargues, Nicolas Gaudenzio
- Scientific Poster – CRS 2021 Virtual Annual Meeting – July 25 to 29, 2021.
December 2020 - Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing
- Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing
- Published in Science Advances – 2020 Dec 4;6(49):eabd9443
- Tejabhiram Yadavalli, Rahul Suryawanshi, Raghuram Koganti, James Hopkins, Joshua Ames, Lulia Koujah, Aqsa Iqbal, Krishnaraju Madavaraju, Alex Agelidis, Deepak Shukla
- Published in Science Advances – 2020 Dec 4;6(49):eabd9443
August 2019 - Near-infrared light activable hydrogels for metformin delivery
- Near-infrared light activable hydrogels for metformin delivery
- Published in Nanoscale – 2019 Aug 29;11(34):15810-15820
- Li Chengnan, Quentin Pagneux, Anna Voronova, Alexandre Barras, Amar Abderrahmani, Valérie Plaisance, Valerie Pawlowski, Nathalie Hennuyer, Bart Staels, Lea Rosselle, Nadia Skandrani, Musen Li, Rabah Boukherroub, Sabine Szunerits
- Published in Nanoscale – 2019 Aug 29;11(34):15810-15820
December 2018 - Poster - A fully functional ex vivo human skin model to study human skin microbiome
- A fully functional ex vivo human skin model to study human skin microbiome
- Scientific Poster presented at the BacTouBac meeting of the Paul Sabatier University in Toulouse – December 11 & 12, 2018
- B. Coupe (Vaiomer), M. Pastore (Genoskin), E. Pagès (Genoskin), E. Braun (Genoskin), A. Broha (Vaiomer), P. Descargues (Genoskin)
- Scientific Poster presented at the BacTouBac meeting of the Paul Sabatier University in Toulouse – December 11 & 12, 2018
January 2018 - Poster - Evaluation of local inflammatory reactions following subcutaneous injection of a pro-inflammatory cocktail in a fully human ex vivo skin model
- Evaluation of local inflammatory reactions following subcutaneous injection of a pro-inflammatory cocktail in a fully human ex vivo skin model
- Scientific Poster presented at DECHEMA Advances in Medical Biology Seminar – January 30 to 31, 2018
- C. Jardet (Genoskin), E. Pagès (Genoskin), E. Raude (Genoskin), F. Seeliger (AstraZeneca), L. Brandén (AstraZeneca), E. Braun (Genoskin), M. Ingelsten (LAAS-CNRS), P. Descargues (Genoskin)
- Scientific Poster presented at DECHEMA Advances in Medical Biology Seminar – January 30 to 31, 2018
