T cell activation in InflammaSkin®
A flow cytometry perspective
Enhanced T cell proliferation and activation in InflammaSkin®: A flow cytometry perspective
Understanding T cell activation and proliferation is essential for studying inflammatory skin diseases such as psoriasis. However, conventional preclinical models often struggle to reproduce the complex interactions between skin-resident immune cells and the inflammatory tissue environment.
In this application note, we explore how InflammaSkin®, Genoskin’s ex vivo human skin model of psoriasis-like inflammation, supports the investigation of T cell-mediated immune responses. By inducing a Th17/Th1-polarized inflammatory environment in native human skin, InflammaSkin® provides a physiologically relevant platform for studying immune activation and evaluating potential therapeutic interventions.
Through a comparative study of InflammaSkin® and HypoSkin®, combining flow cytometry and MANTIS® spatial biology analysis, the application note highlights how researchers can:
- Assess T cell activation through increased expression of CD69 in skin-resident T cells.
- Evaluate T cell proliferation by measuring Ki-67 expression under inflammatory conditions.
- Characterize T cell populations, including changes in CD4+ and CD8+ subsets.
- Investigate immune cell distribution and identify CD4+CD8+ double-positive T cells using spatial biology analysis.
Download the application note to explore the experimental findings and discover how InflammaSkin® supports human-relevant psoriasis research and the evaluation of T cell-targeted therapies.
Please fill in the form below to access the application note.
Comments are closed.