HypoSkin^®

Preserving skin viability is one of the major challenges in developing ex vivo models that accurately mimic the human organ outside the body. To circumvent this limitation, we developed a series of standardized engineering steps to quickly biostabilize human skin biopsies, while preserving an air-liquid interface and the appropriate biomechanical properties of the tissue. The result is HypoSkin^®, a unique live, immunocompetent, and injectable ex vivo human model designed to help drug developers accelerate the selection of viable candidates.

To evaluate HypoSkin^® stability and potential donor-intrinsic changes in global tissue architecture and cellular gene expression profiles over the culture period, a combination of histochemical and single-cell RNA sequencing longitudinal analyses were performed within the same individual donor.